The 2% Chlorhexidine Gluconate/70% Isopropyl Alcohol formulation, applied with gentle friction to help the solution penetrate the first five cell layers, where 80% of microorganisms reside. • Apply - Starting at the incision site, gently press the applicator against the skin until the solution soaks the sponge, apply using repeated up and down, back and forth strokes for at least 30 seconds, before working outwards to the periphery.